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CRISPR diagnosticsScienceDaily
•78% Informative
PUMA enables the detection of RNA with Cas12 nucleases, which naturally target DNA.
PUMA promises a wide range of applications and high accuracy.
The method is based on reprogramming RNA factors, so-called tracrRNAs , which are naturally involved in helping produce guide RNAs used by Cas9 and Cas12.
The new technology represents a novel form of CRISPR diagnostics that enables reliable molecular testing at the point of care.
The research team is already planning its next steps: "Our goal is to achieve a multiplexed readout similar to that of LEOPARD ".
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